MTO may refer to:

• MTO, French mural and graffiti artist, film maker
• MTO, IATA code for Coles County Memorial Airport in Mattoon-Charleston, Illinois
• MTO, National Rail station code for Marton railway station (Middlesbrough), in England
• MTO (video game company), a Yokohama-based video game developer and publisher founded in May 1996
• MTO, medical timeout, for example in professional tennis
• mto, ISO 639-3 code for the Totontepec Mixe language
• Make to order or made to order, a production approach where products are not built until a confirmed order for products is received
• Mario Tennis Open, a 2012 tennis game for the Nintendo 3DS
• Mars Telecommunications Orbiter, a cancelled Mars mission to enhance communications between Earth and spacecraft on or near Mars
• Mars transfer orbit, also known as trans-Mars injection, a spacecraft maneuver to achieve a trajectory to Mars
• Material take off, an engineering and design term
• Media Take Out, an African-American celebrity gossip website
• Mediterranean Theater of Operations, a World War II area designation
• Methanol to olefins, a technology for generating polymer precursors from methanol
• Methylrhenium trioxide, also called methyltrioxorhenium
• Ministry of Transportation of Ontario, a ministry of the government of Ontario
• Munger, Tolles & Olson, a law firm based in California
• Motor Tax Office, a type of government agency comparable to a Department of Motor Vehicle
• Moving to Opportunity, a US federal housing program
• Multimodal transport operator, an enterprise which moves goods using more than one type of transportation
• Medium-Term budgetary Objective, part of the Stability and Growth Pact (SGP) agreement between member states of the European Union
• Music Theory Online, the online journal of the Society for Music Theory